Abstract

Noviany, Ratu Dwi Gustia Rasyidi and Andi Setiawan

DOI : http://dx.doi.org/10.13005/ojc/330124

The aim of this study was to identify and characterize groups of antioxidant active naturally from methanol fraction of the stem of Anredera cordifolia throughantioxidant assay-guided separation. The methanol fraction (MF) displayed the highest antioxidant activity, compared to ethyl acetate fraction (EAF) and hexane fraction(HF) using cyclic voltammetry method. Ascorbic acid was used as a positive control in this research.The repeated fractionation of active MF by MPLC and HPLC analysis yielded a constituent with strong antioxidant property. The active constituent(RNV-1) was predicted as a flavonoid type based on the UV spectrum data. The antioxidant activity results exhibited that RNV-1had a coefficient ofantioxidant activity (K) sligtly higher than K ofascorbat acid with the values of 0.055 and 0.052 respectively.  It was found that RNV-1 demonstrated anexcellent antioxidant activity in comparison with the standard antioxidant activity through antioxidant assays-guided separation. The results obtained suggested that the stem of A. cordifolia can be used potentially asa source of natural antioxidants by contributing beneficial health effects.

Antioxidant assay-guided separation; Anredera cordifolia; binahong; flavonoid; cyclic voltammetry

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