Abstract

Vinita sawaddipanich and Saksit chanthai^*

DOI : http://dx.doi.org/10.13005/ojc/320301

A headspace-single drop microextraction (HS-SDME) is a good choice to analyze volatile and semivolatile compounds in different matrices without any interference of the sample matrix. HS-SDME spends a very little solvent consumption for the determination of volatile aroma compounds (VACs). In this study, some VACs including 2-methylbutyraldehyde, 3-methyl-1-butanol, sec-butyl acetate, 1-octen-3-one and trans,trans-2,4-decadienal were performed with hexadecane as extraction solvent. The parameters affecting the HS-SDME method were investigated in details including extraction temperature, enrichment time, extraction time and sample volume. The optimum conditions were consisted of 45^oC extraction temperature, 15min enrichment, 20min extraction time and 3mL sample volume. Comparison between liquid-liquid extraction (LLE) and HS-SDME for quantitative analysis was carried out using tert-butanol as an internal standard. From results revealed that the recoveries were obtained between 81.06-90.55% for LLE and 80.26-90.09% for HS-SDME. The results demonstrated that the proposed method could be successfully applied for the determination of VACs.

Headspace-single drop microextraction; Gas chromatography; Volatile aroma compounds; Tomato

[ View HTML Full Text]