Abstract

Sohier M.Syame^1,2*, W. S. Mohamed³, Rehab K. Mahmoud⁴ and Shimaa T. Omara¹

DOI : http://dx.doi.org/10.13005/ojc/330632

Development of nanotechnology, nanoparticles based product and its application is generating interest of many researchers due to its promising biological achievement. However, it is well known that inorganic nanomaterials are good antimicrobial agents. Among the various nanoparticles, metal nanoparticles as copper assume special importance due to its low cost and easy availability. In this study, the green synthesis method as eco-friendly approach is used to produce biologically copper oxide nanoparticles from Ficus carica leaf extract, stable CuO NPs were formed. The synthesized nanoparticles is characterized through the UV-Vis Spectrophotometer as it found to be 437 nm, Transmission Electron microscopy (TEM) investigated particle sizes in the range 51-62 nm and typical XRD patterns of the formed CuO NPs with high phase purity were obtained. Chitosan stabilizer as naturally occurring polymers was added to the prepared copper nanoparticles in different amounts to obtain Chitosan-CuO with different CuO percentage for long-term stability, for prevention the agglomeration of nanoparticles and enhancing their antibacterial efficacy. FTIR spectroscopy analysis was performed to copperoxide nanoparticle, chitosan, copperoxide chitosan composite to confirm that CuO nanoparticle was mixed with polymer. The antibacterial efficacy of chitosan, copper nanoparticles alone was studied against 22 bacterial pathogen like Coaggulase +ve S. aureus, methicillin-resistant Coaggulase -ve S. aureus, Klebsiella pneumonia as gram positive bacteria, Escherichia coli, e.coli o157 Salmonella typhi, Pseudomonas aeruginosa as gram negative bacteria that showed antibacterial activity against Gram positive as well as Gram negative bacteria. Antimicrobial activities of polymer/metal composites (Co oxide-chitosan nanoparticle) is studied against the same bacterial pathogen. The effect of the prepared Chitosan-CuO composite on ultrastructure of bacterial cells were evaluated by scanning electron microscopy (SEM), it was found that the antibacterial activity of Cu-chitosan nanoparticle composite is more greater than antibacterial activity of copper nanoparticles and chitosan alone that indicate the addition of chitosan stabilizer enhance at great extent the antimicrobial activity of CuO NPs.

Copperoxide nanoparticle, Copperoxide –Chitosan nanoparticle composite, Ficus Carica leaf extract, Antimicrobial activity.

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